An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers.
نویسنده
چکیده
An analytical anion-exchange procedure has been developed for the rapid separation of acid-soluble nucleotides (the so-called "free" or tissue nucleotides). It permits assay at low pressures (275-415 kPa; 40-60 psi) in less than 1 h on 10-cm columns of Aminex resins (conventional styrene-type anion exchangers) with alkaline citrate solutions as the eluent. Separation variables have been investigated, to determine optimum conditions for the routine analysis of samples containing tissue nucleotides. Also described here is a simple solvent-extraction procedure for removing HCIO4 or CCI3CO2H quantitatively from cell extracts that contain acid-soluble nucleotides: they are removed from aqueous acid solutions with a water-insoluble amine dissolved in a water-immiscible solvent.
منابع مشابه
Analytical Techniques for Cell Fractions. Iii. Nucleotides and Related Compounds.
The methods described in this paper are devised for the separation of the common nucleotides, nucleosides, and purine and pyrimidine bases on one column at one pH with the compounds quantitated by a sensitive automatic technique. Following the classical work of Cohn and co-workers (l-6)) nucleotides have been generally separated on anion-exchange columns at acid pH’s, nucleosides on anion-excha...
متن کاملAnion- and cation-exchange MicroHPLC utilizing poly(methacrylates)-coated monolithic silica capillary columns.
A novel method was developed for the preparation of highly efficient anion- and cation-exchange microHPLC columns using an on-column polymerization of methacrylates having amine or sulfonic acid functional groups onto monolithic silica capillary columns modified with 3-methacryloxypropyltriethoxysilane as the anchor groups. The chromatographic evaluation of the columns using nucleic acids, nucl...
متن کاملDesigning and Constructing Blood Flow Monitoring System to Predict Pressure Ulcers on Heel
Background: A pressure ulcer is a complication related to the need for the care and treatment of primarily disabled and elderly people. With the decrease of the blood flow caused by the pressure loaded, ulcers are formed and the tissue will be wasted with the passage of time.Objective the aim of this study was to construct blood flow monitoring system on the heel tissue which was under external...
متن کاملNew family of high-resolution ion exchangers for protein and nucleic acid purifications from laboratory to process scales.
A new family of polymer-based ion exchangers was tested for the purification of acidic and basic proteins on both the analytical and preparative scales. Protein-Pak HR series packings are available as strong cation (SP) and weak anion (DEAE) exchangers, allowing the development of a purification method regardless of the isoelectric point of protein. Three particle sizes, 8, 15 and 40 microns, a...
متن کاملDesigning an Algorithm for Cancerous Tissue Segmentation Using Adaptive K-means Cluttering and Discrete Wavelet Transform
Background: Breast cancer is currently one of the leading causes of death among women worldwide. The diagnosis and separation of cancerous tumors in mammographic imagesrequire accuracy, experience and time, and it has always posed itself as a major challenge to the radiologists and physicians. Objective: This paper proposes a new algorithm which draws on discrete wavelet transform and adaptive ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 21 9 شماره
صفحات -
تاریخ انتشار 1975